Figure 4.

Mitotic midzone proteins affect microtubule organization and central spindle protein localization in meiotic metaphase I. Oocytes were stained with DNA (blue), Tubulin (green), and Subito or INCENP (red). (A and A′) Wild-type oocytes localize Subito or INCENP to the central region of a bipolar metaphase spindle. (B and B′) Rho1 and (C and C′) sticky RNAi oocytes show disorganized microtubules (marked with arrows) and aberrant Subito or INCENP localization. (D and D′) RhoGEF2 and (E and E′) pbl RNAi oocytes resemble wild type in both microtubule organization and Subito localization. (F) Graph summarizing the spindle defects in wild-type and RNAi oocytes. Significantly different P-values are indicated by asterisks. Bars, 5 µm.