Table 3. Frequency of mono-orientation in oocyte knockouts of central spindle proteins.
| Genotype | AACAC % mono-orientation (n)a | DODECA % mono-orientation (n)b | P-valuec (AACAC) | P-valuec (DODECA) | Total |
|---|---|---|---|---|---|
| Wild type | 4 (2) | 0 | NA | NA | 45 |
| Wild type (HS)d | 5.5 (1) | 5.5 (1) | NA | NA | 18 |
| tum HMS01417 (HS)d | 50 (10) | 45 (9) | 0.004 | 0.009 | 20 |
| Rho1 HMS00375 | 35 (9) | 15 (4) | 0.001 | 0.019 | 26 |
| sticky GL00312 | 27 (6) | 18 (4) | 0.013 | 0.015 | 22 |
| RhoGEF2 HMS01118 | 20 (5) | 13 (3) | 0.045 | 0.039 | 24 |
| pbl GL01092 | 0 | 0 | NS | NS | 15 |
| polo GL00014 | 61.9 (13) | 47.6 (10) | 0.009 | 0.01 | 21 |
a
Percentage of total oocytes with second chromosome AACAC probe mono-oriented.
b
Percentage of total oocytes with third chromosome Dodeca probe mono-oriented.
c
Fisher’s exact test was used to calculate the P-values compared to wild type.
d
HS = heat shock: These values were obtained from independent experiments with the heat-shock driver.