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. 2016 Jan 5;10(1):e0004306. doi: 10.1371/journal.pntd.0004306

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© 2016 Romano et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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Fig 1 — A) Gating strategy for accurate determination of the proportion of regulatory T cells in PBMCs 1. Lymphocytes; 2. CD4⁺ T cells; 3. CD4⁺CD45RA⁺FOXP3^low T cells; 4. CD4⁺CD45RA^-FOXP3^low T cells; 5.CD4⁺CD45RA^-FOXP3^high T cells); B) Percentage of blood CD45RA^-FOXP3^low non-Tregs (gray bars), CD45RA^-FOXP3^high T cells (black bars) and CD45RA⁺FOXP3^low T cells (white bars) among the blood CD4⁺ T cells of infected patients (group 1; n = 76); C) Proportion of CD45RA^-FOXP3^high T cells and CD45RA⁺FOXP3^low T cells expressing CD25, CCR7, CXCR3, CCR5, or CTLA-4 among blood CD4⁺ T cells in 45 patients (excluding splenectomized patients). A nonparametric test was used. * p<0.01.