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A673 cells were infected with lentiviruses expressing FLI-1-EWS shRNA-1 (F-E #1) or luciferase shRNA and were selected with 2 μg/ml puromycin for 2 days. Two million cells were subcutaneously injected into SCID mice and four weeks after injection, tumor volume was determined using a caliper (5 mice/group).
