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. 2015 Nov 4;15(11):27954–27968. doi: 10.3390/s151127954

Table 2.

Ct values with annealing time of 15, 10, and 9 s, respectively, denaturation and extension time of 30 and 40 s, respectively.

Annealing Time (s)
15 10 9
Ct (Mean ± SD) 32.2 ± 0.49 32.3 ± 0.51 32.9 ± 0.68