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. 2015 Nov 4;15(11):27954–27968. doi: 10.3390/s151127954

Table 3.

Ct values extension time of 15, 10, 5, and 4 s, respectively, with denaturation and annealing time of 30 s, respectively.

Extension Time (s)
15 10 5 4
Ct (Mean ± SD) 32.2 ± 0.48 32.3 ± 0.50 32.3 ± 0.51 33.2 ± 0.67