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(A) Relative Fluorescence (RF) changes in the presence of ATP (100 μM), GTP (100 μM), CTP (100 μM), and UTP (100 μM), respectively; (B) RF changes in the presence of ATP (10 μM), ATP (10 μM) + GTP (100 μM), ATP (10 μM) + CTP (100 μM), and ATP (10 μM) + UTP (100 μM), respectively. RF = FT − FB, where FT is the fluorescence reading of a test mixture and FB is the fluorescence reading of the blank mixture. Each data point represents the average value of three independent experiments.
