Table 3. Reciprocal crosses in A. flavus in which single-strain sclerotia were incubated on sterilized soil inoculated with conidia.
Sclerotia on soila | Conidia in sterilized soila | ||||||
---|---|---|---|---|---|---|---|
MAT1-1 | MAT1-2 | MAT1-1 | MAT1-2 | No. sclerotia examined | % fertile sclerotiab | No. progenyc | |
29537 | × | 29536 | 480 | 1.1 ± 0.9d | 36 | ||
29536 | × | 29537 | 300 | 98.7 ± 0.6d | 36 | ||
29537 | × | 29507 | 300 | 0 | |||
29536 | × | AF36 | 300 | 0 | |||
29507 | × | 21882 | 300 | 96.7 ± 2.3e | 35 | ||
21882 | × | 29507 | 300 | 1.0 ± 0.0e | 36 | ||
29507 | × | 29473 | 300 | 0 | |||
21882 | × | 29536 | 300 | 0 | |||
29473 | × | AF36 | 300 | 85.7 ± 0.6f | 36 | ||
AF36 | × | 29473 | 300 | 27.7 ± 12.5f | 36 | ||
29473 | × | 29537 | 300 | 0 | |||
AF36 | × | 21882 | 300 | 0 |
aStrain numbers (except AF36) from Agricultural Research Service Culture Collection (NRRL), Peoria, Illinois; mating-type designations from Ramirez-Prado et al. [12]; vegetative compatibility group designations are shown in Table 1. Biocontrol strains include NRRL 21882 (from Afla-Guard) and AF36 (= NRRL 18543).
bSclerotia with ascospores considered fertile; means ± SD based on three soil cups.
cProgeny obtained from three ascocarps in separate sclerotia (n = 11–12 progeny per ascocarp).
d,e,fReciprocal crosses within each pair significantly different (P < 0.0001) according to chi-square test of independence (S1 Text).