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. 2016 Jan 5;11(1):e0146169. doi: 10.1371/journal.pone.0146169

Table 3. Reciprocal crosses in A. flavus in which single-strain sclerotia were incubated on sterilized soil inoculated with conidia.

Sclerotia on soila Conidia in sterilized soila
MAT1-1 MAT1-2 MAT1-1 MAT1-2 No. sclerotia examined % fertile sclerotiab No. progenyc
29537 × 29536 480 1.1 ± 0.9d 36
29536 × 29537 300 98.7 ± 0.6d 36
29537 × 29507 300 0
29536 × AF36 300 0
29507 × 21882 300 96.7 ± 2.3e 35
21882 × 29507 300 1.0 ± 0.0e 36
29507 × 29473 300 0
21882 × 29536 300 0
29473 × AF36 300 85.7 ± 0.6f 36
AF36 × 29473 300 27.7 ± 12.5f 36
29473 × 29537 300 0
AF36 × 21882 300 0

aStrain numbers (except AF36) from Agricultural Research Service Culture Collection (NRRL), Peoria, Illinois; mating-type designations from Ramirez-Prado et al. [12]; vegetative compatibility group designations are shown in Table 1. Biocontrol strains include NRRL 21882 (from Afla-Guard) and AF36 (= NRRL 18543).

bSclerotia with ascospores considered fertile; means ± SD based on three soil cups.

cProgeny obtained from three ascocarps in separate sclerotia (n = 11–12 progeny per ascocarp).

d,e,fReciprocal crosses within each pair significantly different (P < 0.0001) according to chi-square test of independence (S1 Text).