Table 1.
Summary of otoferlin expression and quantification of afferent presynaptic marker RIBEYE (mean±SD per OHC) in Wild Type (WT) and Pit1dw mice.
| Age | P5 | P9 | P14 | P42 | P14 (+TH) |
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Marker/Region | A | M | A | M | A | M | A | M | A | M |
| Otoferlin (WT) | + | + | − | − | − | − | − | − | NA | NA |
| Otoferlin (Pit1dw) | + | + | + | + | + | + | + | − | + | + |
| RIBEYE (WT) | 13.4 ± 2.8 | 23 ± 3.3 | 9.9 ± 1.6 | 3.0 ± 1.4 | 3.3 ± 1.0 | 2.6 ± 0.6 | 3.2 ± 0.4 | 2.8 ± 0.2 | NA | NA |
| RIBEYE (Pit1dw) | 16.4 ± 1.6 | 19.3 ± 1.2 | 14.0 ± 1.0 | 12.6 ± 2.6 | 12.8 ± 2.3 | 6.2 ± 1.6 | 5.2 ± 0.5 | 2.6 ± 0.3 | 3.3 ± 0.6 | 1.9 ± 0.4 |
Presence of otoferlin immunoreactivity, and quantification of presynaptic marker, RIBEYE, is shown for P5, P9, P14, P42, for both WT and Pit1dw mice, and at P14 following TH-treatment from P3-P4 for Pit1dw mice. Data from the apical and mid turns of the cochlea are shown. + : Present; - : Absent; A : Apical Turn; M : Mid Turn; TH : Thyroid Hormone (P3-P4); NA : Not Applicable