Table 5. Observed and calculated complex dissociation half-life.

Complex half-life⊥		20°C	25°C	30°C
PfAMA1-F*PfRON2sp1	Kd (nM)	31.0 ± 2	43 ± 3	61.0 ± 5
PfAMA1-F*PfRON2sp1	Experimental	1641 s (27.4 min)	900 s (15.0 min)	554 s (9.2 min)
PfAMA1-F*PfRON2sp1	Calculated	1730 s (28.8 min)	960 s (16.0 min)	544 s (9.1 min)
ΔDII-PfAMA1-PfRON2sp1	Kd (nM)	60.0 ± 5	94.0 ± 5	125 ± 10
ΔDII-PfAMA1-PfRON2sp1	Experimental	100 s (1.6 min)	48 s (0.8 min)	30 s (0.5 min)
ΔDII-PfAMA1-PfRON2sp1	Calculated	166 s (2.8 min)	69.9 s (1.2 min)	30 s (0.5 min)

^⊥ Observed complex half-life, t_1/2 = ln2 /k_{off slower} [39]