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. 2016 Jan 5;11(1):e0146031. doi: 10.1371/journal.pone.0146031

Fig 2. p21 stimulates the poly(ADP-ribosyl)ation activity of PARP-1 in the presence of nicked DNA.

Fig 2

(A) Crosslinking assay of PARP1 activation in the presence (+) or absence (−) of p21. Photoreactive 5’-[32P]-labeled 0.1 μM DNA*pF (lanes 1–4) or DNA*p (lanes 5–8) were incubated with 0.1 μM PARP1 with (+) or without (−) NAD+. (B,C) Kinetic analysis of PARP-1 activity in the presence of p21. 0.2 μM nicked DNApF (B), or DNAp (C) was incubated with 60 nM PARP-1, 100 μM NAD+ + 0.5 μCi [32P]-NAD+ in the presence of 1.2 μM p21 (where indicated). The data are mean values ± S.D., n = 3.