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. 2016 Jan 5;11(1):e0146663. doi: 10.1371/journal.pone.0146663

Fig 2. Cell-associated HIV crosses the A2EN epithelial barrier and is enhanced by CT infection.

Fig 2

A2EN cells were infected with CT serovar D (> 95% infection rate) for 72 h. 1 x 106 HIVBaL-infected MT4-R5 cells were added to the apical chambers of mock and CT-infected A2EN cells for 3 h at 37°C. (A) Viral RNA in basolateral supernatants from cell-associated HIV migration assays was quantitated using real-time PCR. Bars represent the mean RNA copies ± SD. (B) Basolateral supernatants collected in cell-associated HIV migration assays were incubated with uninfected MT4-R5 cells for 3 d. Supernatants from exposed MT4-R5 cells were then assayed for infectious virus using TZM-bl cells. Controls included TZM-bl cells incubated with an equivalent volume of epithelial cell medium. Data is expressed as a percentage of the mean RLU obtained for the medium alone controls. Bars represent the mean percentages ± SD. Results shown are representative of 4 separate experiments, each performed in quadruplicate. *p < 0.05.