Fig 6. Basolateral supernatants from CT-infected A2EN cells enhance HIV infection of PBMC.

A2EN cells grown on cell culture inserts were infected with CT serovar D for 72 h. (A, C) Apical and (B, D) basolateral supernatants collected from mock or CT-infected A2EN cells were incubated with (A, B) resting PBMC for 4 d or (C, D) PHA-activated PBMC for 2 d. The PBMC were then infected with 200 TCID₅₀ of HIV_BAL. Activated PBMC were additionally cultured with A2EN supernatants for another 2 d after infection. Every 2 d after infection, the culture medium was completely replaced. The viral content in culture medium collected 6 d after infection was determined using a p24 ELISA. Presented are the cumulative p24 results from 3 separate experiments, each performed in triplicate. Bars represent the mean ± SD. *p < 0.05.