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. 2016 Jan 5;11(1):e0146287. doi: 10.1371/journal.pone.0146287

Fig 8. Effect of resveratrol in in vitro virulence of E. histolytica trophozoites and in E. invadens encystment.

Fig 8

A) Ingested erythrocytes after different incubation times with untreated or resveratrol -treated (110 μM, 12 h and washing out) trophozoites. Values represent the mean ± standard error of three independent experiments. **p<0.01, ***p<0.001. B) Representative images of trophozoites with ingested erythrocytes at different incubation times. C) Destruction of MDCK cell monolayers incubated with untreated or resveratrol-treated (110 μM, 12 h and washing out) trophozoites. Bottom: representative images of MDCK monolayers incubated with trophozoites and stained with methylene blue. C: MDCK cells that were not in contact with trophozoites. 0.4% ethanol-treated trophozoites were used as positive controls. Values represent the mean ± standard error of three independent experiments. *p<0.05,** p<0.01,*** p<0.001. D) Cysts formed were counted under the epifluorescence microscope and number of cysts in untreated cells was taken as 100%. Values represent the mean ± standard error of three independent experiments., ** p<0.01,***p<0.001.