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. 2015 Nov 23;126(1):50–67. doi: 10.1172/JCI80761

Figure 12. Tgsm/p22phox aortic homogenates prime DCs to promote T cell proliferation and activation.

Figure 12

(A) Experimental design. Aortic homogenates from WT or tgsm/p22phox mice were used to prime naive DCs, which were then cocultured with T cells from these mice in various combinations. (B, C, F, and G) Flow cytometry and bar graphs identifying live CD3+ T cells and CD4+/CD8+ T cell subsets from cocultured DCs and T cells. (D, E, H, and I) Representative flow cytometry images and bar graphs showing percentages and numbers of proliferating CD4+ (D and H) and CD8+ (E and I) T cells, as determined using CSFE dilution (n = 6, 1-way ANOVA). Homo, homogenates. Black lines indicate original data, orange peaks indicate initial population, and purple peaks indicate generations of cells undergoing division as simulated by FlowJo 7.6.4 software. *P < 0.05 compared with Tgsm/p22phox homogenate + Tgsm/p22phox T cells; **P < 0.01 Tgsm/p22phox homogenate + Tgsm/p22phox T cells.