Figure 3. Flow cytometry analysis of inflammatory cells in the aorta of WT and tg^sm/p22phox mice.

Single cell suspensions were prepared from freshly isolated mouse aortas via enzymatic digestion and mechanical dissociation. Live cell singlets were analyzed for vascular inflammatory cells. (A–H) CD45⁺ total leukocytes, F4/80⁺ macrophages, CD19⁺ B lymphocytes, CD3⁺ T lymphocytes, and CD4⁺/CD8⁺T cell subsets were identified in the aorta of 9-month-old WT (A–D) and tg^sm/p22phox mice (E–H). (I–N) Quantification of infiltrating leukocyte subsets using 2-way ANOVA (n = 6–8). **P < 0.01 vs. Tg^sm/p22phox; ^#P < 0.05; ^##P < 0.01.