Figure 4. CDK4 represses lipolysis and is a positive modulator of lipogenesis.

(A) Quantification of triglyceride content of eWAT from 23-week-old Cdk4^+/+ and Cdk4^nc mice (n = 3). (B) Quantification of triglyceride content of eWAT from 27-week-old Cdk4^+/+ and Cdk4^R24C/R24C mice (n = 3). (C) Rate of NEFA release in eWAT explants from 23-week-old fasting Cdk4^+/+ and Cdk4^nc mice (n = 3). (D) Rate of NEFA release in eWAT explants from 28-week-old fasting Cdk4^+/+ and Cdk4^R24C/R24C mice (n = 6). (E) Ex vivo lipogenesis experiments in eWAT explants using labeled ¹⁴C acetate incorporation to detect TG, DG, and PL synthesis of 22-week-old Cdk4^+/+ and Cdk4^nc mice (n = 3). (F) Ex vivo lipogenesis experiments in eWAT explants of 27-week-old Cdk4^+/+ and Cdk4^R24C/R24C mice. ¹⁴C-acetate incorporation was used to detect TG, DG, and PL synthesis by TLC (n = 3). Data are expressed as mean ± SEM. Statistically significant differences were determined with unpaired 2-tailed Student’s t tests. *P < 0.05.