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. 2015 Nov 30;126(1):123–136. doi: 10.1172/JCI81108

Figure 6. Brain endothelial LRP1 substantially mediates Aβ clearance in vivo.

Figure 6

(A) 5.14 nM [125I] Aβ1–42 and 40 μCi/ml [14C]-inulin, a paracellular marker, were microinfused into brain ISF of the caudate nucleus. Efflux was studied at designated time points by determining remaining radioactivity in the brain (n = 5, n = 5, n = 3, n = 3, n = 4, n = 5 mice per group from left to right). (B) No alteration was observed in the bulk flow clearance of [14C]-inulin (n = 5 mice per group). (C) Efflux across the BBB of 5.14 nM [125I] Aβ1–42 15 minutes after microinfusion in brain ISF demonstrated the substantial contribution of LRP1 (n = 5 mice per group). (D and E) Scarce presence of tracers in CSF 15 minutes after microinjection into the caudate nucleus (n = 3 mice per group). (F) Contribution of brain endothelial LRP1 to total and BBB clearance of 5.14 nM [125I] Aβ1–42 within 15 minutes (n = 5 mice per group). Data represent mean ± SEM. For statistical analyses, unpaired t test was used. *P < 0.05, **P < 0.01.