Figure 9. Preferential clearance of Aβ_1–40 species by brain endothelial LRP1.

Contrasting the Aβ_1–42/Aβ_1–40 ratio in 5xFAD Lrp1_BE^–/– and 5xFAD Lrp1_BE^fl/fl Aβ pools demonstrates a differential clearance of Aβ species. Densitometry analysis of immunoprecipitated soluble brain Aβ, insoluble brain Aβ, and plasma Aβ with 6E10 antibody from 7-month-old mice (n = 3, n = 5, n = 7, n = 6, n = 3, n = 3 from left to right) showed higher Aβ_1–40 and Aβ_1–42/Aβ_1–40 ratios in plasma and lower and Aβ_1–42/Aβ_1–40 ratios in brain fractions when LRP1 is present in brain endothelial cells. Data represent mean ± SEM. For statistical analyses, unpaired t test was used. *P < 0.05, **P < 0.01, ***P < 0.001.