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. 2015 Dec 14;126(1):389–401. doi: 10.1172/JCI83831

Figure 5. Progesterone, but not BMP6 or IL-6, requires SFK activity to increase hepcidin gene expression.

Figure 5

qPCR for hepcidin gene expression in HepG2 cells serum starved for 18 hours and then preincubated with the SFK inhibitor PP2 (10 μM) or control (DMSO) for 30 minutes and then exposed to (A) progesterone (30 μM for 8 hours), (B) BMP6 (20 ng/ml for 3 hours), or (C) IL-6 (100 ng/ml for 3 hours). #P < 0.001 compared with control treated cells exposed to vehicle, †P < 0.001 compared with control treated cells exposed to progesterone, 2-way ANOVA (n = 4 per group). All results expressed as mean ± SEM.