Figure 2. HDL from AgNO₃-injected SAA-DKO mice retains part of its antiinflammatory effect on adipocytes.

HDL was isolated from the plasma of AgNO₃- or PBS-injected C57BL/6 and SAA-DKO mice. 3T3-L1 adipocytes were treated as described in the legend to Figure 1. Saa3, Ccl2, Il1β, and Il6 gene expression (A), plasma membrane cholesterol content (B), cholesterol efflux (C), LR content (D), ROS generation (E), and TLR4 translocation to LRs (F) were analyzed. An antibody to caveolin 1 (CAV1) was used to stain LRs. Fractions 7–9 contain LRs, and fractions 1 –4 are non–LR-containing fractions. Data represent mean ± SD. Data are representative of at least 3 independent experiments. *P < 0.001 vs. C57BL/6 (AgNO₃) HDL, **P < 0.001 vs. C57BL/6 (control) HDL. ANOVA and Bonferroni post-hoc test.