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. 2015 Dec 7;126(1):266–281. doi: 10.1172/JCI83475

Figure 7. HDL from moderately inflamed obese mice loses its antiinflammatory effect on adipocytes and colocalizes with ECM in AT.

Figure 7

(A) 3T3-L1 adipocytes were preexposed for 6 hours to the indicated concentrations of HDL, which was isolated from the plasma of Ldlr−/− mice fed chow or HFHSC diets for 20 weeks (n = 5). After that, adipocytes were treated as per the legend to Figure 1 for measurement of Saa3, Ccl2, Il1β, and Il6 gene expression. (B) Epididymal fat isolated from C57BL/6 mice fed chow or HFHSC diets for 20 weeks was stained with Movat’s pentachrome stain and analyzed by IHC using anti–apoA-I and anti-SAA antibodies (B, n = 9). SAA colocalized with apoA-I in the ECM (blue stain with Movat’s) of ATs from mice fed the HFHSC diet. Tissues were photographed using microscopy. Original magnification, ×60. Data represent mean ± SD. *P < 0.001 vs. chow. ANOVA and Bonferroni post-hoc test for A, and Student t test for B.