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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Obesity (Silver Spring). 2015 Nov 5;23(12):2349–2356. doi: 10.1002/oby.21198

Figure 2.

Figure 2

Metabolic measurements during each protocol day

Metabolic rate (kcal/day) was measured using indirect calorimetry with a validated ventilated hood system (TrueOne 2400 metabolic cart, Parvo Medics, Sandy, UT, USA). Before each measurement day, a flow meter calibration was conducted and before each use, the metabolic cart was calibrated with reference gas. After achieving steady state (5 minutes, denoted with the dotted line), expired gases were collected for 10 minutes and used to calculate metabolic rate. Trained technicians monitored subjects and instructed them to keep their eyes open to ensure they remained awake during the tests. Data shown as mean ± SD.