Fig. 1.

Experimental design. For each of 1060 chemicals, the chemical exposure at 6 hpf saw one of 5 concentrations of a given chemical added to individual embryo wells in 96-well plates. At 24 hpf, plates were subjected to light pulse exposure (at 30 s and 40 s) for the PMR assay. Movement was recorded across the short (<1 min) interval and then converted into the concentration–response profile shown under behavioral assessment. At 5 dpf, plates were assessed for specific developmental malformations, an example of which is shown under developmental assessment