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. 2016 Jan 5;6(1):10. doi: 10.1007/s13205-015-0315-4

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Molecular analysis of T₀ Transgenic rice plants. a A 800 bp internal sequence of cry2AX1 gene was amplified by PCR from the DNA isolated from putative transgenic plants. Lanes 1 and 21, 100 bp marker; Lanes 2–17, Putative transgenic plants of ASD16; Lane 18, Non transformed control plant; Lane 19, Negative control (water); Lane 20, pUH-rtp-2AX1 plasmid as a positive control. b Southern blot hybridization analysis of T₀ transgenic rice plants expressing cry2AX1 gene. DNA sample isolated from transgenic and non-transgenic plants digested with HindIII restriction enzyme, DNA fragments separated by electrophoresis, transferred to nylon membrane and allowed to hybridize with a radioactively labelled 800 bp internal sequence of cry2AX1 gene. M, λ/HindIII marker; Lanes 1–16, Genomic DNA from transgenic rice plants UR1, UR2, UR3, UR4, UR5 UR6, UR7, UR8, UR9, UR10, UR11, UR12, UR13, UR14, UR15, UR16, respectively; Lane 17, Non-transformed control plant; Lane 18, Positive control (pUH-rtp-2AX1)