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. 2016 Jan 5;6(1):10. doi: 10.1007/s13205-015-0315-4

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — a RT-PCR analysis of rice actin (housekeeping gene) in T₁ transgenic plants. Lanes 1, 100 bp ladder; Lanes 2–6, transgenic plants (UR11–1, UR11–2, UR11–3, UR11–4 and UR11–7); Lane 7, non-transgenic plants (Wild type ASD16). b RT-PCR analysis of cry2AX1 in T₁ transgenic plants. Lane 1 and 9, 100 bp ladder; Lanes 2–6, transgenic plants (UR11–1, UR11–2, UR11–3, UR11–4 and UR11–7); Lane 7, non transgenic plants (Wild type ASD16), Lane 8, Positive control (pUH-rtp-2AX1). c The temporal expression of Cry2AX1 protein in T₁ transgenic rice plants. The Cry2AX1 protein concentration in fresh leaf of T₁ transgenic plants at vegetative, tillering and reproductive stage