Fig. 4. Immunoblotting detection of IRF-3 and IRF-7 in PBMCs from healthy and PI cattle. (A) Representative panels of IRF-3 and IRF-7 are shown. The expression of (B) IRF-3 and (C) IRF-7. Protein lysates were prepared with protease inhibitors and the expression of IRF-3 and IRF-7 was analyzed by immunoblotting, detected with enhanced chemiluminescence, and then analyzed with the Quantity One densitometric software. Simultaneous staining of β-actin on the same membrane was used as a control for protein loading in the individual lanes. Results are presented as the ratio of the intensity of the IRF-3 or IRF-7 band to the intensity of the β-actin band. Data presented are the means ± SEM (n = 6 per group). P < 0.05 is considered statistically significant.