Table 1.
Ligands of HLA-E*01:03 derived from HLA−/TPN+ LCL 721.221
| HLA-E*01:03 | ||
|---|---|---|
| Sequence | Length | Origin |
| HAVSEGTKAVTKYTSSK | 17 | Histone H2B type 1-L |
| PAETATPAPVEKSPAKK | 17 | Histone H1.5 |
| AYVRLAPDYDALDVANK | 17 | 60S ribosomal protein L23a |
| HAVSEGTKAVTKYTSAK | 17 | Histone H2B type 1-J |
| AVSDGVIKVFNDMKVRK | 17 | Cofilin-1 |
| HAVSEGTKAVTKYTSS | 16 | Histone H2B type 1-L |
| QLLQANPILEAFGNAK | 16 | Myosin-9 |
| KSADTLWDIQKDLKDL | 16 | l-lactate dehydrogenase B chain |
| AYVRLAPDYDALDVAN | 16 | 60S ribosomal protein L23a |
| HAVSEGTKAVTKYTSA | 16 | Histone H2B type 1-J |
| TGLIKGSGTAEVELKK | 16 | Pyruvate kinase isozymes M1/M2 |
| VSDGVIKVFNDMKVRK | 16 | Cofilin-1 |
| ASGNYATVISHNPETK | 16 | 60S ribosomal protein L8 |
| TAEILELAGNAARDNK | 16 | Histone H2A type 1-D |
| HAVSEGTKAVTKYTSA | 16 | Histone H2B type 1-J |
| PAPVEKSPAKKKATK | 15 | Histone H1.5 |
| SADTLWDIQKDLKDL | 15 | l-lactate dehydrogenase B chain |
| TGLIKGSGTAEVELK | 15 | Pyruvate kinase isozymes M1/M2 |
| KSADTLWGIQKELQF | 15 | l-lactate dehydrogenase A chain |
| HGSYEDAVHSGALND | 15 | T-complex protein 1 subunit alpha |
| SDGVIKVFNDMKVRK | 15 | Cofilin-1 |
| AGNLGGGVVTIERSK | 15 | 60S ribosomal protein L22 |
| AQAAAPASVPAQAPK | 15 | 60S ribosomal protein L29 |
| PRKIEEIKDFLLTAR | 15 | 60S ribosomal protein L38 |
| SEGTKAVTKYTSSK | 14 | Histone H2B type 1-L |
| VLKQVHPDTGISSK | 14 | Histone H2B type 1-L |
| SWTAADTAAQITQR | 14 | HLA class I histocompatibility antigen, Cw-1 alpha chain |
| FISVGYVDDTQFVR | 14 | HLA class I histocompatibility antigen, Cw-1 alpha chain |
| NIDDGTSDRPYSHA | 14 | 60S ribosomal protein L27 |
| VLKQVHPDTGISSK | 14 | Histone H2B type 1-J |
| RKTVTAMDVVYALK | 14 | Histone H4 |
| SADTLWGIQKELQF | 14 | l-lactate dehydrogenase A chain |
| ASAETVDPASLWEY | 14 | Fascin |
| TVVNKDVFRDPAL | 13 | 60S ribosomal protein L27 |
| KTVTAMDVVYALK | 13 | Histone H4 |
| EGIPALDNFLDKL | 13 | Elongation factor 2 |
| RVTIMPKDIQLAR | 13 | Histone H3.3C |
| PVAVMAESAFSFK | 13 | COP9 signalosome complex subunit 8 |
| QTVAVGVIKAVDK | 13 | Elongation factor 1-alpha 1 |
| ILELAGNAARDNK | 13 | Histone H2A type 1-D |
| GTGASGSFKLNK | 12 | Histone H1.5 |
| KQVHPDTGISSK | 12 | Histone H2B type 1-J |
| VGGTSDVEVNEK | 12 | 60 kDa heat shock protein, mitochondrial |
| NSVVEASEAAYK | 12 | 14-3-3 protein eta |
| ALRYPMAVGLNK | 12 | 60S ribosomal protein L36 |
| SLVSKGTLVQTK | 12 | Histone H1.5 |
| PELAKSAPAPK | 11 | Histone H2B type 1-L |
| SEMEVQDAELK | 11 | Proliferation-associated protein 2G4 |
| QTYSTEPNNLK | 11 | 60S ribosomal protein L28 |
| PMFIVNTNVPR | 11 | Macrophage migration inhibitory factor |
| AGFAGDDAPR | 10 | Actin, cytoplasmic 1 |
| RVNAGTLAVL | 10 | von Willebrand factor A domain-containing protein 8 |
| IGQSKVFFR | 9 | Myosin-9 |
sHLA-E*01:03 molecules were purified from LCL 721.221 and bound peptides sequenced. Given is the sequence and origin of the peptide fragment. Peptide length ranged from 9 to 17 AAs