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. 2015 Oct 15;157(1):336–345. doi: 10.1210/en.2015-1709

Figure 5.

Figure 5.

Western blot analysis and densitometric ratios of IGF-1R downstream signaling. A, Immunoblot and densitometric analysis of the following: IGF-1R (B), phosphorylated Akt (C), phosphorylated ERK (D), phosphorylated JNK (E), and phosphorylated p38 (F) in hearts from WT and CIGF1RKO mice (n = 3–4 per group). Data are presented as the mean ± SEM. Two-way ANOVA was performed to analyze differences by age (young vs very old) and genotype (WT vs CIGF1RKO). *, P < .05, ***, P < .001 vs young WT; †, P < .05 vs young CIGF1RKO. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VO, very old; Y, young.