Figure 7.

CREB activation does not increase in response to short-term LH treatment in GCs from WT or Epab^−/− mice. Western bot analysis was performed to compare LH-induced phosphorylation of CREB in GCs from WT and Epab^−/− mice. GCs were collected from WT and Epab^−/− mice at 12 weeks of age 44–48 hours after 5-IU PMSG injection, cultured, serum starved, and treated with LH (1 μg/mL) for 5 and 10 minutes. Representative Western blottings of total (T) and phosphorylated (p) CREB are shown in A. The intensity of Western blot bands was analyzed using densitometry (B). The ratio of p-CREB to total CREB was normalized to untreated WT control. Data are presented as mean ± SEM from 3 separate experiments. Phosphorylation of CREB was not significantly different in WT or Epab^−/− GCs after 5 or 10 minutes of LH treatment.