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. 2015 Nov 10;157(1):268–281. doi: 10.1210/en.2015-1690

Figure 2.

Figure 2.

Constitutively activated AMPK impaires osteoblast differentiation. A, LacZ- or AMPK T172D-overexpressing cells were stained by Alizarin Red on the indicated day after DM exposure. B, Lysates obtained from the cells expressing LacZ or AMPK T172D on the indicated day following DM exposure were immunoblotted with antiosteocalcin or β-actin. C, AMPK T172D-overexpressing cells were stained with Alizarin Red on 21 days after DM exposure in the absence (control) or the presence of compound C (1uM) that was added on the indicated day. D, Lysates obtained from cells overexpressing AMPK T172D after 21 days of DM exposure in the absence or in the presence of compound C (1uM) added on the indicated day were immunoblotted with antiosteocalcin or β-actin. Each bar is the ratio of the scan value of the osteocalcin band divided by the β-actin band. **, P < .01 indicates significant differences between two treatments.

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