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. 2015 Nov 10;157(1):268–281. doi: 10.1210/en.2015-1690

Figure 5.

Figure 5.

Activation AMPK is suppressed at the middle and late stage of differentiation via activation of mTOR and AKT. A, Cell lysates from MC-3T3 cells following exposure to DM for the indicated days were immunoblotted with an anti-pmTOR (S2448), pAKT (S473), pAMPK (S485), or β-actin. B and C, Lysates from LacZ- (Ctrl) or IGFBP-2-overexpressing cells (B) or from MC-3T3 cells expressing AMPK T172D (C) obtained on the indicated day after DM exposure were immunoblotted with anti-pmTOR (S2448), pULK (S757), pAKT (S473), pAMPK (S485), or AMPK. D, Lysates from MC-3T3 cells following exposure to DM for 9 days in the presence of or absence of AKT inhibitor (AKT in) (500nM) were immunoblotted with an anti-pAKT (S473), pAMPK (S485, T172), or AMPK. E, Lysates from MC-3T3 cells following exposure to DM for 9 days in the presence of or absence of rapamycin (500nM) were immunoblotted with an anti-pmTOR (S2448), pULK (S757, S555), or β-actin.

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