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. 2015 Oct 14;157(1):346–357. doi: 10.1210/en.2015-1630

Figure 1.

Figure 1.

Confirmation of cardiovascular-specific 11β-HSD1 deletion. Hsd11b1 mRNA and 11β-HSD1 protein were assessed by real-time PCR (normalized to β-actin housekeeping gene) and Western blotting (normalized to β-actin loading control), respectively, in heart, aorta, liver, and skeletal muscle from Hsd11b1fl/flSm22α-Cre+ (Hsd11b1CVCre+) and control Hsd11b1fl/flSm22α-Cre (Hsd11b1CVCre−) mice. Expression in hsd11b1CVCre+ is expressed relative to maximum achieved in tissue from Hsd11b1CVCre−; ***, P < .001; **, P < .01 vs parallel control; n = 4/group.