Table 1.
qPCR primers used to evaluate FUM gene expression by Fusarium verticillioides in maize grains.
| Locus | Primer sequence (5′–3′) | Fragment size (bp) | Reference/NCBI accession number |
|---|---|---|---|
|
FUM1F FUM1R |
GAGCCGAGTCAGCAAGGATT AGGGTTCGTGAGCCAAGGA |
90 | Lopez-Errasquín et al., 2007 |
|
FUM3F FUM3R |
CTTGGCGGTGCCCATACTA GGACCAAGAGCGTGGATG |
60 | This study/AF155773 |
|
FUM6F FUM6R |
GATAGACTCGGGGCTGAGA AGCTCGCCGACAGAATC |
100 | This study/AF155773 |
|
FUM7F FUM7R |
CATCGTATCTACATTGTCGCATC TGTACTCTCCAACAATATGAATGAGTC |
100 | This study/AF155773 |
|
FUM8F FUM8R |
CAACAGAAATACGCAATGACG TGCTCGACCACTACATCAGG |
99 | This study/AF155773 |
|
FUM13F FUM13R |
GCCTTTGGTCTTGTTCTCTCA CGTCAATTATTGCCTCTTTCAA |
100 | This study/AF155773 |
|
FUM14F FUM14R |
TAGGTCCAGGTCGAGATGCT GGAAGCCAAGAACCCAATCT |
99 | This study/AF155773 |
|
FUM15F FUM15R |
TGCCATCCAGAATGACGATA GAGTCTCAGGAGAGCGAGGA |
94 | This study/AF155773 |
|
FUM19F FUM19R |
ATCAGCATCGGTAACGCTTATGA CGCTTGAAGAGCTCCTGGAT |
88 | Lopez-Errasquín et al., 2007 |
|
TUBF TUBR |
CCGGTATGGGTACTCTGCTC CTCAACGACGGTGTCAGAGA |
95 | Lopez-Errasquín et al., 2007 |
|
CALMF CALMR |
ACGGTTTCATTTCTGCTGCT TCAGCCTCTCGGATCATCTC |
97 | This study/AF155773 |