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. 2016 Jan 6;5:101. doi: 10.3389/fcimb.2015.00101

Figure 1.

Figure 1

Greater virulence and mortality of Cryptococcus gattii JP02 infection compared to Cryptococcus neoformans H99 infection and independence from CD4 cell-mediated immunity. (A) Shorter survival of mice infected with JP02 compared with H99-infected mice (n = 8). Some mice were depleted of CD4-positive cells by an anti-CD4 injection before infection. The survival from H99 infection was shorter in CD4-depleted mice compared with that in CD4-positive control mice, while CD4 depletion had no effect on the survival from JP02 infection. (B) Fungal burden was substantially higher in the lungs of JP02-infected mice compared with the lungs of H99-infected mice. Results are representative of two individual experiments with n = 5 for each experiment (means ± standard deviations, SD). (C) Capsule formation as evaluated by India ink staining of infected lung homogenates showing thick capsule formation by both species. Bar, 10 μm. Survival curves were compared by log-rank test and the means of fungal burden were compared by the unpaired t-test. *P < 0.01; NS, not significant.