Figure 3.
Lower IL-6 release from JAWSII dendritic cells in response to live or heat-killed JP02 cells compared with live or heat-killed H99 cells. JAWSII cells (2 × 105 cells/mL) were incubated with live (MOI = 1) or heat-killed (MOI = 5) cryptococcal cells for 24 h, and supernatant was obtained for ELISA. (A) Incubation with live cells. (B) Incubation with heat-killed cells. H99 + JP02 means co-incubation with heat-killed H99 and heat-killed JP02 cells (MOI = 5, each). The experiments were performed in triplicate (means ± SD). Treatment group means were compared by the unpaired t-test, except for vs. H99 in (B), which were compared by the Mann–Whitney U-test. *P < 0.01; NS, not significant.