Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 6;6:1481. doi: 10.3389/fmicb.2015.01481

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Kortman, Dutilh, Maathuis, Engelke, Boekhorst, Keegan, Nielsen, Betley, Weir, Kingsbury, Kluijtmans, Swinkels, Venema and Tjalsma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Toxicity of the microbial metabolome for an intestinal monolayer. Effect of dialysis liquids (before exchange in TIM-2; not containing microbial metabolites) and dialysates (after exchange in TIM-2; at 72 h) of the lowFe condition and 50FeS condition on the integrity and permeability of a Caco-2 monolayer. Epithelial integrity is displayed as relative transepithelial electrical resistance (TEER) which was regularly recorded during 7 h incubation. HBSS was used as a standard maintaining buffer which should keep the TEER at a constant high level. The decrease in epithelial integrity of the 50FeS dialysate was significantly faster compared to the lowFe dialysate as analyzed by linear regression. We note that iron was removed from the dialysis liquids and dialysates prior to applying them to the epithelial monolayers (A). Epithelial permeability as determined by the flux of phenol red from the apical side to the basolateral side, analyzed by an unpaired t-test (B). ^**p < 0.01 n = 3 for the dialysis liquids before exchange in TIM-2 and n = 6 for the dialysates after exchange; at 72 h in TIM-2.