Table 1.

Oligonucleotides used for SEP3^(75-178) mutagenesis.

Mutant	Oligonucleotides
M150A	5′-CTCTCAGGACACAGTTTGCGCTTGACCAGCTCAAC-3′
	5′-GTTGAGCTGGTCAAGCGCAAACTGTGTCCTGAGAG-3′
L154A	5′-CAGTTTATGCTTGACCAGGCGAACGATCTTCAGAGTAAGG-3′
	5′-CCTTACTCTGAAGATCGTTCGCCTGGTCAAGCATAAACTG-3′
L171A	5′-CTGACTGAGACAAATAAAACTGCAAGACTAAGGTTAGCTGATGG-3′
	5′-CCATCAGCTAACCTTAGTCTTGCAGTTTTATTTGTCTCAGTCAG-3′
SpliceΔ161-174	5′-CCAGCTCAACGATCTTCAGAGTAAGCTAGCTGATGGATGAGAGACAAATAAAACTCTAAGACTAAGG-3′ (forward)
	5′-CCTTAGTCTTAGAGTTTTATTTGTCTCTCATCCATCAGCTAGCTTACTCTGAAGATCGTTGAGCTGG-3′ (reverse)
Y98K	5′-CTTAGTAGCCAGCAGGAGAAGCTCAAGCTTAAGGAGCG-3′
	5′-CGCTCCTTAAGCTTGAGCTTCTCCTGCTGGCTACTAAG-3′
Y105N	5′-CTCAAGCTTAAGGAGCGTAATGACGCCTTACAAAGAACCC-3′
	5′-GGGTTCTTTGTAAGGCGTCATTACGCTCCTTAAGCTTGAG-3′
L115R	5′-CAAAGAACCCAAAGGAATAGGTTGGGAGAAGATCTTGGACCTC-3′
	5′-GAGGTCCAAGATCTTCTCCCAACCTATTCCTTTGGGTTCTTTG-3′
L131V	5′-CTAAGTACAAAGGAGCTTGAGTCAGTTGAGAGACAGCTTGATTC-3′
	5′-GAATCAAGCTGTCTCTCAACTGACTCAAGCTCCTTTGTACTTAG-3′
L135M	5′-CTTGAGTCACTTGAGAGACAGATGGATTCTTCCTTGAAGC-3′
	5′-GCTTCAAGGAAGAATCCATCTGTCTCTCAAGTGACTCAAG-3′
L135A	5′-GTCACTTGAGAGACAGGCTGATTCTTCCTTGAAGC-3′
	5′-GCTTCAAGGAAGAATCAGCCTGTCTCTCAAGTGAC-3′