Table 2.
Effect of point mutations on SEP3 oligomerisation.
| SEP3(75-178) constructs | Oligomerisation state | |
|---|---|---|
| Tetramerisation interface | Wild type | Tetramer/dimer |
| (Puranik et al., 2014) | M150A | Dimer |
| L154A | Dimer | |
| L171A | Dimer | |
| SpliceΔ161-174 | Dimer | |
| Dimerisation interface | Y98K | Dimer/monomer |
| (this study) | Y105N | Tetramer/monomer |
| L115R | Unstable complex | |
| L131V | Unstable complex | |
| L135M | Dimer/monomer | |
| L135A | Unstable complex | |
Point mutations targetting the highly conserved residues involved in the putative dimerisation and tetramerisation interface of SEP3 were chosen for mutational analysis. Oligomeric state was determined by size exclusion chromatography. Where two states exist, the predominant species is marked in bold. “Unstable complex” is used to denote a complex mixture of species between monomeric, dimeric, and tetrameric states with no predominance for a particular oligomerisation state.