Figure 5.

Immunostaining of different intracellularly filled and functionally characterized muscle afferents from ex vivo recording in Naive, BAO Control, and PenY1+BAO conditions. A–D, Example of a characterized muscle afferent from a Naive mouse that responded only to low metabolites. This cell was negative for ASIC3 and P2X3. E–H, Another cell from a Naive mouse that responded to mechanical and heat stimuli in addition to high metabolites was positive for ASIC3 but not P2X3. I–L, A cell from a mouse with BAO that responded not only to mechanical stimulation but also to low and high mixtures of metabolites was positive for both ASIC3 and P2X3. M–T, A cell from a P2Y1-knockdown animal after ischemic injury that responded to mechanical stimulation and also to the high mixture of metabolites was found to be positive for ASIC3 and P2X3 (M–P), while another cell from this condition that only responded to the low-metabolite mixture was also immunopositive for ASIC3 but not P2X3 (Q–T). U, V, Positive (U) and negative (V) immunohistochemistry controls show specific binding of ASIC3 antibody.