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. 2016 Jan 6;36(1):19–30. doi: 10.1523/JNEUROSCI.2856-15.2016

Figure 6.

Figure 6.

Selective knockdown of P2Y1 in muscle afferents does not alter the de novo expression of P2X3 or ASIC3 in DRGs after BAO but does reduce the membrane expression of ASIC3. A–C, The average number of cells per section that were positive for P2X3 (A) and ASIC3 (B) and that coexpressed ASIC3 and P2X3 (C) was significantly increased during ischemia (BAO and PenCON+BAO) compared with Naive, but this was not reversed by the P2Y1 knockdown (PenY1+BAO). Kruskal–Wallis with Dunn's post hoc; *p < 0.02 vs Naive. D, Examples of immunostaining in DRGs from Naive, BAO, and PenY1+BAO conditions. E, The membrane protein fraction isolated from DRGs of ischemic mice (BAO and PenCON+BAO) shows increased expression of ASIC3 versus Naive mice; inhibition of P2Y1 partially blocked this increase in membrane-bound ASIC3 in DRGs during BAO (one-way ANOVA with Holm–Sidak post hoc; **p < 0.02 vs Naive and PenY1+BAO; *p < 0.03 vs Naive and p = 0.069 vs PenY1+BAO). F, Examples of DRG membrane fraction Western blots for ASIC3 in Naive, BAO, PenCON+BAO, or PenY1+BAO mice.