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In Neuro-2a cells not expressing nAChRs, nicotine induced no significant changes in nuclear versus cytoplasmic localization of ATF6. A, Representative confocal images of Neuro-2a cells transfected alone with ATF6-eGFP were treated with no drug, nicotine (100 nm), Tu (100 nm), or nicotine with Tu. B, Normalized ATF6-eGFP nuclear translocation. These data were analyzed with a one-way ANOVA and Tukey comparisons. n.s., Not significant.
