FIGURE 1.
CuZn-SOD1 (SOD1) activity and expression in tissues of experimental mice. In-gel activity staining for SOD1 activity in total protein extracts obtained from skeletal muscles (A), neuronal tissues (B), liver, and kidney (C) (upper). Results of Western blot analysis of SOD1 in the same protein extracts are shown in (middle). Blots were also re-probed with monoclonal anti-tubulin or anti-β-actin antibodies from mouse as a loading control (lower). Data are representative for three independent Western blot analyses performed using extracts from 3 mice of each experimental group. The intensity of the SOD1 bands (relative to the intensity of tubulin or actin bands) was quantified with a Molecular Imager using Quantity One software (Bio-Rad) and is shown in arbitrary units to present protein level (Supplementary Table). Results are expressed as mean ± SD for three mice in each experimental group. Significant differences (P < 0.01) in SOD1 protein levels were found between transgenic (SOD1 and SOD1G93A) and wild-type mice in a given tissue and age group.