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Histological examination of iron loading in the gastrocnemius muscle of 4-month-old experimental mice. Non-heme iron deposits were detected by staining with Perls Prussian Blue in the gastrocnemius muscle of wild-type (A) and SOD1 mice (B). Blue staining (indicated by arrows) was present exclusively in the muscle section of symptomatic SOD1G93A mice. Staining of muscle sections from three SOD1G93A mice are shown (C,D,E).
