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. 2016 Jan 6;6:1483. doi: 10.3389/fmicb.2015.01483

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Copyright © 2016 Ambroset, Coluzzi, Guédon, Devignes, Loux, Lacroix, Payot and Leblond-Bourget.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of ICEs and dICE belonging to the Conj_Tn916 superfamily. (A) Relaxases; (B) coupling proteins; (C) VirB4 proteins. Bootstrap supports are given as followed: ML/BioNJ. X marks the nodes that are not validated with BioNJ. ICE names are colored according to the family of conjugation module they encode: orange = Conj_Tn916, green = Conj_ICESt3 (including elements encoding relaxases related to the one of ICE_SmuA159_tRNAleu in bold dark green). Because of their very close phylogenetic relationships, relaxases (A), CP (B), and VirB4 (C) of only three ICEs representative of the Conj_Tn916 family are shown. Refer to Supplementary Table S1 for ICE/dICE and strain details.