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. 2016 Jan 1;30(1):18–33. doi: 10.1101/gad.267757.115

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© 2016 Ferretti et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — BMI1 induces the invasive signature with concomitant nuclear accumulation of β-catenin. (A–H) qPCR analysis on total RNA from A375 (A–D) and MA2 (EH) cells confirmed the differential expression of representative genes involved in EMT (A–E), TGFβ (B–F), noncanonical Wnt/PKC (C–G), and EGF/PDGF (D–H) pathways in CTL versus BMI1 cells. Results shown are mean ± SD. (I) Expression of selected proteins evaluated by Western blot analysis of CTL and BMI1 MA2 cells. (J) Subcellular fractionation and Western blot analysis of CTL and BMI1 A375 and MA2 cells, with HSP90 and Lamin A/C as control for cytoplasmic and nuclear fractions. See also Supplemental Figure S5.