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. 2016 Jan 6;6:1501. doi: 10.3389/fmicb.2015.01501

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Copyright © 2016 Slamti, Lemy, Henry, Guillot, Huillet and Lereclus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hemolytic activity of ΔcodY cells over-expressing oppA and oppA-like-encoding genes. Cells were patched onto a Columbia sheep blood agar plate supplemented with xylose and grown at 37°C for 16 h. The cells either harbor the empty vector p304-Pxyl+ or overexpress our gene of interest under the control of a xylose-inducible promoter. wt, Bt 407- (p304-Pxyl+); ΔcodY, Bt ΔcodY (p304-Pxyl+); (02170), Bt ΔcodY (p304-Pxyl+-02170); (06720), Bt ΔcodY (p304-Pxyl+-06720); (09790), Bt ΔcodY (p304-Pxyl+-09790); (12330), Bt ΔcodY (p304-Pxyl+-12330); (12390), Bt ΔcodY (p304-Pxyl+-12390); (20960), Bt ΔcodY (p304-Pxyl+-20960); (36620), Bt ΔcodY (p304-Pxyl+-36620); (36650), Bt ΔcodY (p304-Pxyl+-36650); (36660), Bt ΔcodY (p304-Pxyl+-36660). This picture is representative of at least three independent experiments.