Table 1.
Plasmids used this study.
| Name | Relevant features | Reference |
|---|---|---|
| pRN-ΔcodY | codY flanking regions were amplified by PCR from the chromosome of Bt 407- using primer pairs codYUp-HindIII/codYUp-XbaI and codYDn-XbaI/codYDn-BamHI and cloned between the BamHI and HindIII restriction sites of the thermosensitive plasmid pRN5101 (Villafane et al., 1987) to generate a 504 bp internal deletion in codY | This study |
| p1618K-Pxyl | Replicative multicopy vector harboring the xylose-inducible promoter of xylA | Dubois et al., 2013 |
| p1618K-Pxyl’-codY | Transcription of codY is driven by the xylose-inducible promoter of xylA | Dubois et al., 2013 |
| p304-Pxyl+ | PxylA was amplified by PCR from the chromosome of Bacillus subtilis strain 168 using primer pair Pxyl1/PxylRBS+ and cloned between the SphI and XbaI sites of the replicative multicopy pHT304 plasmid (Arantes and Lereclus, 1991). p304-Pxyl+ harbors a modified version of the xylose-inducible promoter region of xylA to enhance translation efficiency (Stammen et al., 2010) | This study |
| p304-Pxyl+’-plcR | plcR was amplified by PCR from the chromosome of strain Bt 407- using primer pair SP1/PO2 and cloned between the NcoI and BamHI restriction sites of p304-Pxyl+ | This study |
| p304-Pxyl+’-papR7i | Primers PapR7i1 and PapR7i2 were annealed as previously described (Slamti and Lereclus, 2002) and the resulting double-stranded DNA fragment encoding the ADLPFEF peptide was cloned between the NcoI and EcoRI restriction sites of p304-Pxyl+ | This study |
| p304-Pxyl+’-papRFL | papRFL was amplified by PCR from the chromosome of strain Bt 407- using primer pair PapRFL1/PapRFL2 and cloned between the NcoI and EcoRI restriction sites of p304-Pxyl+ | This study |
| pPplcR’-Z | The promoter region of plcR was amplified by PCR from the chromosome of strain Bt 407- using primer pair Pp3/Pp2 and cloned between the HindIII and BamHI restriction sites of the multicopy replicative vector pHT304.18-lacZ (Agaisse and Lereclus, 1994). The promoter of plcR drives the transcription of the reporter gene lacZ | This study |
| pPplcA’-Z | The promoter of plcA drives the transcription of lacZ in pHT304.18-lacZ | Lereclus et al., 1996 |
| pPpapR’-Z | The promoter of papR drives the transcription of lacZ in pHT304.18-lacZ | Agaisse et al., 1999 |
| pMAD-ΔoppA | oppA flanking regions were amplified by PCR from the chromosome of Bt 407- using primer pairs LSoppA1/LSoppA2 and LSoppA3/LSoppA4 followed by overlap extension PCR and cloned between the EcoRI and BamHI restriction sites of the thermosensitive plasmid pMAD (Arnaud et al., 2004) | This study |
| p304-Pxyl+’-02170/06720/09790/12330/12390/20960/36620/36650/36660 | The gene corresponding to each of the indicated locus tags was amplified by PCR from the chromosome of strain Bt 407- using primer pair c02170.1/c02170.2, c06720.1/c06720.2, c09790.1/c09790.2, c12330.1/c12330.2, c12390.1/c12390.2, c20960.1/c20960.2, c36620.1/c36620.2, c36650.1/c36650.2 or c36660.1/c36660.2, and cloned between the NcoI and KpnI restriction sites of p304-Pxyl+ | This study |