Table 2.
Strains used this study.
| Name | Relevant features | Reference |
|---|---|---|
| Bt 407- | Acrystalliferous Bacillus thuringiensis strain 407 | Lereclus et al., 1989 |
| Bt ΔcodY (p1618K-Pxyl’-codY) | Strain deleted for codY with a copy of the gene in trans | This study |
| Bt (p1618K-Pxyl’-codY) | Wild-type strain with a copy of codY in trans | This study |
| Bt ΔcodY | Strain deleted for codY, obtained by curing the p1618K-Pxyl’-codY plasmid from strain Bt ΔcodY (p1618K-Pxyl’-codY) | This study |
| Bt ΔcodY (p1618K-Pxyl’-codY)B | Bt ΔcodY transformed with p1618K-Pxyl’-codY to complement the mutation | This study |
| Bt ΔcodY (pPplcR’-Z) | Bt ΔcodY in which we measure the activity of the promoter of plcR | This study |
| Bt ΔcodY (pPplcA’-Z) | Bt ΔcodY in which we measure the activity of the promoter of plcA | This study |
| Bt ΔcodY (pPpapR’-Z) | Bt ΔcodY in which we measure the activity of the promoter of papR | This study |
| Bt (pPplcR’-Z) | Bt 407- in which we measure the activity of the promoter of plcR | This study |
| Bt (pPplcA’-Z) | Bt 407- in which we measure the activity of the promoter of plcA | This study |
| Bt (pPpapR’-Z) | Bt 407- in which we measure the activity of the promoter of papR | This study |
| Bt ΔcodY (p1618K-Pxyl’-codY)B (pPplcA’-Z) | Bt ΔcodY transformed with p1618K-Pxyl’-codY to complement the mutation in which we measure the activity of the promoter of plcA | This study |
| Bt ΔcodY (p1618K-Pxyl’) (pPplcA’-Z) | Bt ΔcodY transformed with p1618K-Pxyl’ in which we measure the activity of the promoter of plcA | This study |
| Bt (p1618K-Pxyl’) (pPplcA’-Z) | Bt 407- transformed with p1618K-Pxyl’ in which we measure the activity of the promoter of plcA | This study |
| Bt ΔcodY (p304-Pxyl+’-plcR) | Bt ΔcodY in which we express plcR from the xylose-inducible promoter of xylA | This study |
| Bt ΔcodY (p304-Pxyl+) | Bt ΔcodY carrying the empty p304-Pxyl+ vector | This study |
| Bt (p304-Pxyl+) | Bt 407- carrying the empty p304-Pxyl+ vector | This study |
| Bt ΔplcR | Strain Bt 407- in which plcR has been replaced with the aphA3 gene | Salamitou et al., 2000 |
| Bt ΔplcR (p304-Pxyl+’-plcR) | Bt ΔplcR in which we express plcR from the xylose-inducible promoter of xylA | This study |
| Bt ΔcodY (p304-Pxyl+’-papR7i) | Bt ΔcodY in which we express papR7i from the xylose-inducible promoter of xylA. The mature 7 aa form of PapR is produced directly inside the cells | This study |
| Bt ΔcodY (p304-Pxyl+’-papRFL) | Bt ΔcodY in which we express papRFL encoding the full-length form of PapR (including the signal sequence) from the xylose-inducible promoter of xylA | This study |
| Bt ΔpapR PplcA’-lacZ | Strain Bt 407- in which papR has been replaced with the aphA3 gene and carrying a chromosomal transcriptional fusion between the promoter region of plcA and the reporter gene lacZ | Slamti and Lereclus, 2002 |
| Bt ΔpapR PplcA’-lacZ (p304-Pxyl+’-papR7i) | Strain Bt ΔpapR PplcA’-lacZ in which we express papR7i from the xylose-inducible promoter of xylA. The mature 7 aa form of PapR is produced directly inside the cells | This study |
| Bt ΔpapR PplcA’-lacZ (p304-Pxyl+’-papRFL) | Strain Bt ΔpapR PplcA’-lacZ in which we express papRFL encoding the full-length form of PapR (including the signal sequence) from the xylose-inducible promoter of xylA | This study |
| Bt ΔpapR | Strain Bt 407- in which papR has been replaced with the aphA3 gene using pRNΔpapR::KanR (Slamti and Lereclus, 2002) | This study |
| Bt ΔpapR (pPplcA’-Z) | Bt ΔpapR in which we measure the activity of the promoter of plcA | This study |
| Bt ΔoppA | Bt 407- carrying a markerless deletion of oppA made with pMAD-ΔoppA | This study |
| Bt ΔcodY (p304-Pxyl+’-02170/06720/09790/12330/12390/20960/36620/36650/36660) | Strains Bt ΔcodY in which we express each gene corresponding to the indicated locus tags from the xylose-inducible promoter of xylA | This study |