Table 2.
Sequence number of the EST in Nicotiana benthamiana corresponding to the targeted proteins and the primers used in the construction of TRV2 derivatives.
| Targeted protein | EST ID1 | Primers for pTRV2-based construct (5′→3′)2 | PCR product (bp) |
|---|---|---|---|
| DEAD-box ATP dependent RNA helicase 7-like | CN748199 | FP: TTCCGGAATTCCGATAGTGTCATACCCGCATTTA RP: TTCCGCTCGAGCAAGTCCTCAGCAGATACATCAA |
319 |
| GO608259 | FP: TTCCGGAATTCCATTTGTTTTGCCCATATTAGAGTC RP: TTCGGCTCGAGACTGAAAAGAATTGTTTGAACTTGG |
419 | |
| ATP-dependent DNA helicase Q-like 2-like | GO601475 | FP: TTCCGGAATTCAATTCTCAACAAACTCCCTCACA RP: TTCCGCTCGAGGTCTATAATCCGGTCGAAAATCA |
459 |
| CK283590 | FP: TTCCGGAATTCCCAGCATGTGGAAATTGTCTCG RP: TTCGGCTCGAGAGCATTCGCAGCGGTAAAGTAG |
403 | |
| RNA helicase | CK286108 | FP: TTCCGGAATTCTGATTGTTTATTTAGCATGGGATTT RP: TTCCGCTCGAGAAACAGAAGGTTCAATACCCTCTTC |
354 |
| CK282547 | FP: TTCCGGAATTCGACAGCTTATTAAGATCCTTCAGCA RP: TTCCGCTCGAGAGCTAGCAAGAACAACAAACATTCT |
317 | |
| Exoribonuclease (XRN4) | EH369301 | FP: TTCCGGAATTCGCTATTGATGGAGTGGCTCCTAGA RP: TTCGGCTCGAGACCCTCACCAGGAACATTAGCAT |
311 |
| MAP kinase phosphatase-like protein | GO612773 | FP: TTCCGGAATTCTTTGTGCACTGCTACCAAGGAGTGT RP: TTCCGCTCGAGATTCTAAGCACAGAACTCGGACT |
212 |
| Disease resistance protein RGA2 | CK290351 | FP: TTCCGGAATTCGCTGTAGGTTTATCTTTTGATGAT RP: TTCCGCTCGAGGATTCTCCATTCCTTCTATTCTT |
467 |
| Ripening-related protein | ES886818 | FP: TTCCGGAATTCCACTTTCTGTTCACAACTTATTGC RP: TTCGGCTCGAGCTCATACATAATTGTCCAAGTAGTC |
410 |
| Pleiotropic drug resistance like protein | CK291986 | FP: TTCCGGAATTCATGTTGTATACTCCGTTGACATGC RP: TTCGGCTCGAGGGGTCCAAGCTCAACAAGTTCC |
404 |
| Scarecrow-like protein 5 | CN744167 | FP: TTCCGGAATTCTCCAGAAGTCACTAAGGCTATGC RP: TTCGGCTCGAGGAAAACCTGCCATCATGAACCTG |
416 |
| ClpA regulatory subunit of Clp protease complex | GO604713 | FP: TTCCGGAATTCTGATGAAGCTGGTTCTCGTGTTC RP: TTCGGCTCGAGCGTCGAATGGCACGACTAATGG |
411 |
| vacuolar H+-ATPase B subunit | GO601484 | FP: TTCCGGAATTCTTCGGTTGGGAGATGGAACTACT RP: TTCCGCTCGAGATAGCAGCAAAGACAATGGCAAA |
493 |
| NADP+-dependent isocitrate dehydrogenase | EX534040 | FP: TTCCGGAATTCCTTATCTTTCCCTTTGTGGAGTT RP: TTCCGCTCGAGATCTTTTCATCCTTCCCTTCTGGTA |
407 |
| S-adenosylmethionine synthetase | CK290599 | FP: TTCCGGAATTCATTTACCTCCGAGTCTGTGAACG RP: TTCGGCTCGAGCTGTGGCATACCCGAACATGTG |
380 |
| Respiratory burst oxidase homolog | CK292677 | FP: TTCCGGAATTCACCATCATTCGGACACAGAGATAAT RP: TTCGGCTCGAGAAGCATTTCGAACAAGTGAAGATCC |
330 |
1The entity of expressed sequence tag (EST) in N. benthamiana was obtained through search using tBASLTn.
2Nucleotide sequences of forward primer (FP) and reverse primer (RP) used in the PCR to obtain the cDNA fragments for the construction of pTRV2 derivatives. The underlined sequences are engineered restriction sites.